Feng Shen, He Zhang, Miaomiao Wan et al. (10 total)
2025-5-2
Nature Communications Vol. 16 (1)
10.1038/s41467-025-59336-7
摘要
Abstract In plants, endoreplication, the process where nuclear DNA replicates in the absence of mitosis, and remodeling of the primary cell walls are both coupled with cell expansion. However, the mechanisms by which these two processes coordinate to determine cell size remain largely elusive. Here, employing the tcpΔ7 septuple mutant disabling seven of the eight CIN-TCP transcription factors in Arabidopsis, we find that hindered endoreplication progression in tcpΔ7 whereby ploidy increases from...
The research investigates how endoreplication and cell wall remodeling coordinate to determine cell size in plants. Specifically, it examines the mechanisms controlling the progression of endoreplication beyond the 8C ploidy level.
The study uses the tcp Δ 7 mutant in Arabidopsis, which disables seven CIN-TCP transcription factors. Genetic analysis was performed on PGL1 in both wild type and tcp Δ 7 backgrounds to assess pectin's role.
Hindered endoreplication progression in tcp Δ 7 correlates with increased cell wall pectin. CIN-TCPs activate PGL1, which down-regulates pectin abundance and molecular mass. Pectin reduction promotes endocycle progression and cell enlargement.